<scp>Genome‐wide</scp> estrogen receptor β chromatin binding in human colon cancer cells reveals its tumor suppressor activity

Colorectal cancer (CRC) is the third leading cause of death in western world. In women, menopausal hormone therapy has been shown to reduce CRC incidence by 20%. Studies demonstrate that estrogen activating receptor beta (ERβ) protects against CRC. ERβ a nuclear regulates gene expression through interactions with chromatin. This molecular mechanism is, however, not well characterized colon. Here, we present for first time, cistrome different colon cell lines. We use lines engineered express ERβ, optimize and validate an antibody chromatin-immunoprecipitation (ChIP), perform ChIP-Seq. identify key binding motifs, including ERE, AP-1, TCF sites, determine enrichment cis-regulatory chromatin sites genes involved tumor development, migration, adhesion, apoptosis, Wnt signaling pathways. compare corresponding cistromes breast find they are conserved about genes, GREB1, but tethering KLF family motifs characteristic exemplify upregulation putative suppressor CST5 where cells binds regions nearby (−351 bp) transcriptional start site. Our work provides foundation understanding action prevention.